Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae, wild-type enzyme and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, expressed in Saccharomyces cerevisiae, are less stable than wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
tRNAAsp | aspartyl-tRNA synthetase as a component of the multisynthetase complex or recombinant native enzyme | Rattus norvegicus | |
0.0006 | - |
tRNAAsp | tRNAAsp, , truncated enzymes AspRS-DELTAN36 and AspRS-DELTAN20 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Rattus norvegicus | - |
wild-type and two truncated forms lacking 20 or 36 amino acid residues from their amino-terminal polypeptide extension, overexpressed in Saccharomyces cerevisiae | - |
Purification (Comment) | Organism |
---|---|
wild-type and two truncated forms | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.395 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Mammalia | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Rattus norvegicus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme occurs in the multienzyme complex of aminoacyl-tRNA synthetases | Mammalia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus |