Protein Variants | Comment | Organism |
---|---|---|
SerRSDELTA35-97 | truncated mutants with a deletion of the N-terminal arm of the enzyme: SerRSDELTA35-97 and SerRSDELTA56-72. Both mutant have lost their specificity for tRNASer and charge also non-cognate type 1 tRNAs. The deletion has no effect on the amino acid activation step of the reaction, but reduces aminoacylation activity dramatically | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000068 | - |
ATP | seryl-AMP formation, wild-type enzyme | Escherichia coli | |
0.00034 | - |
Ser | seryl-AMP formation, wild-type enzyme | Escherichia coli | |
0.00056 | - |
tRNASer | aminoacylation, wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and truncated mutants with a deletion of the N-terminal arm of the enzyme: SerRSDELTA35-97 and SerRSDELTA56-72 | - |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Escherichia coli | AMP + diphosphate + L-seryl-tRNASer | - |
? |