Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Lenhard, B.; Filipic, S.; Landeka, I.; Skrtic, I.; Söll, D.; Weygand-Durasevic, I.
    Defining the active site of yeast seryl-tRNA synthetase. Mutations in motif 2 loop residues affect tRNA-dependent amino acid recognition (1997), J. Biol. Chem., 272, 1136-1141.
    View publication on PubMed
Search for more literature for 6.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information mutants with mutagenesis of portion of the SES1 gene encoding the motif 2 loop reavel elevated Km values for Ser and ATP accompanied by decreases in kcat Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.004
-
 ATP ATP-diphosphate exchange Saccharomyces cerevisiae
0.01 0.048 ATP ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
0.025
-
 ATP aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.06 0.55 ATP aminoacylation, mutant enzymes Saccharomyces cerevisiae
0.063
-
 Ser aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.17 0.7 Ser aminoacylation, mutant enzymes Saccharomyces cerevisiae
0.45
-
 Ser serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae
0.45 0.89 Ser serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 wild-type and mutants with mutagenesis of a portion of the SES1 gene encoding the motif 2 loop
-
Thermus thermophilus P34945
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Saccharomyces cerevisiae AMP + diphosphate + L-seryl-tRNASer
-
 ?
ATP + L-serine + tRNASer
-
 Thermus thermophilus AMP + diphosphate + L-seryl-tRNASer
-
 ?
additional information serine-dependent ATP-diphosphate exchange Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03 0.55 Ser aminoacylation, mutant-enzymes Saccharomyces cerevisiae
0.031 0.51 ATP aminoacylation, mutant enzymes Saccharomyces cerevisiae
0.04 1.97 ATP serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
0.052 2.1 Ser serine-dependent ATP-diphosphate exchange, mutant enzymes Saccharomyces cerevisiae
0.057 0.65 tRNASer aminoacylation, mutant enzymes Saccharomyces cerevisiae
0.97
-
 Ser aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.99
-
 ATP aminoacylation, wild-type enzyme Saccharomyces cerevisiae
1.1
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae
3.5
-
 ATP serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae
3.8
-
 Ser serine-dependent ATP-diphosphate exchange, wild-type enzyme Saccharomyces cerevisiae