Cloned (Comment) | Organism |
---|---|
the seryl-tRNA synthetase expression vector pET15bmMbSerRs is utilized, SerRS proteins are expressed in Escherichia coli cells | Methanosarcina barkeri |
Protein Variants | Comment | Organism |
---|---|---|
K141A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
K79A | mutated enzyme, significant drop in the serylation rate | Methanosarcina barkeri |
K87A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
K88A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
K90A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
N142A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
R143A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
R38A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
R76A | mutated enzyme, serylation ability completely lost | Methanosarcina barkeri |
R78A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
R94A | mutated enzyme, significant drop in the serylation rate | Methanosarcina barkeri |
Y89A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Methanosarcina barkeri | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography on nickel-nitrilotriacetc acid-agarose and cation exchange chromatography on a Resource S column | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methanogenic-type seryl-tRNA synthetase | - |
Methanosarcina barkeri |
mMbSerRS | - |
Methanosarcina barkeri |
SerRS | - |
Methanosarcina barkeri |
Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
aminoacylation assay | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
aminoacylation assay | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanosarcina barkeri |