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Literature summary for 6.1.1.11 extracted from
- Misacylation of pyrrolysine tRNA in vitro and in vivo (2008), FEBS Lett., 582, 3353-3358.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Methanosarcina barkeri str. Fusaro |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
contains two serS genes that encode two different types of SerRSs: a bacterial-type SerRS present in most organisms, and a highly divergent SerRS only found in some methanogenic archaea | - |
| Methanosarcina barkeri str. Fusaro | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified by Ni-NTA chromatography and SDS gel electrophoresis | Methanosarcina barkeri str. Fusaro |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + Fusaro tRNAPyl | Methanosarcina barkeri Fusaro tRNApyrrolysine (tRNAPyl) can be misacylated with serine by the Methanosarcina barkeri bacterial-type seryl-tRNA synthetase in vitro and in vivo in Escherichia coli. Compared to the Methanosarcina barkeri Fusaro tRNA, the Methanosarcina barkeri MS tRNAPyl contains two base changes: a G3:U70 pair | Methanosarcina barkeri | AMP + diphosphate + L-seryl-Fusaro tRNAPyl | - |
? |  |
| ATP + L-serine + tRNAPyl | - |
Methanosarcina barkeri str. Fusaro | AMP + diphosphate + L-seryl-tRNAPyl | - |
? | |
| additional information | in contrast to the bacterial-type SerRS the methanogenic SerRS shows no detectable charging of tRNApyrrolysine (tRNAPyl) | Methanosarcina barkeri | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterial-type SerRS | - |
Methanosarcina barkeri |
| methanogenic SerRS | - |
Methanosarcina barkeri |
| SerRS | two different types: bacterial-type SerRS and highly divergent SerRS | Methanosarcina barkeri str. Fusaro |
| Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
| Seryl-tRNA synthetase | - |
Methanosarcina barkeri str. Fusaro |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Methanosarcina barkeri |
| 7.2 | - |
aassay at | Methanosarcina barkeri |
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Release 2023.1 (January 2023)
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