Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated mutants lacking the tRNA-binding domain, deletion of the C-terminal tRBD of MetRS-Ce results in a 10fold increase in the kcat of Met-tRNAMet formation and a 15fold increase in KM for tRNAMe compared to the wild-type enzyme | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Caenorhabditis elegans | MetRS specifically binds tRNAMet and catalyzes the synthesis of methionyl-tRNAMet. The C-terminal appended domain causes a slow release of aminoacyl-tRNA and establishes a limiting step in the global aminoacylation reaction | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Caenorhabditis elegans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | MetRS specifically binds tRNAMet and catalyzes the synthesis of methionyl-tRNAMet. The C-terminal appended domain causes a slow release of aminoacyl-tRNA and establishes a limiting step in the global aminoacylation reaction | Caenorhabditis elegans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | MetRS is a multidomain protein, structural organization: the very C-terminal appended domain is related to the oligonucleotide binding-fold-based tRNA-binding domain recovered at the C-terminus of MetRS from plant, but, in the nematode enzyme, this domain is separated from the core enzyme by an insertion domain | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Caenorhabditis elegans |
MetRS | - |
Caenorhabditis elegans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
evolution | MetRS from nematode is functionally related to human MetRS despite the fact that their appended tRNA-binding domains have distinct structural folds, and are not orthologues | Caenorhabditis elegans |