Literature summary for 6.1.1.10 extracted from

Larson, E.T.; Kim, J.E.; Zucker, F.H.; Kelley, A.; Mueller, N.; Napuli, A.J.; Verlinde, C.L.; Fan, E.; Buckner, F.S.; Van Voorhis, W.C.; Merritt, E.A.; Hol, W.G.
Structure of Leishmania major methionyl-tRNA synthetase in complex with intermediate products methionyladenylate and pyrophosphate (2011), Biochimie, 93, 570-582.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the His-tagged catalytic core of MetRS in Escherichia coli using the expression vector AVA0421	Leishmania major

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged catalytic core of methionyl-tRNA synthetase, containing a 3C protease cleavage site, in complex with the substrates MgATP2- and methionine, and in complex with two products, methionyladenylate and pyrophosphate, along with a Mg2+ ion that bridges them, hanging drop vapor diffusion, room temperature, 0.002 ml of 22 mg/ml protein in 25 mM HEPES, pH 7.0, 0.5 M NaCl, 0.025% sodium azide, 5% glycerol, 1 mM TCEP, 0.01 mM ZnCl2, 10 mM MgATP2- and 10 mM L-methionine, is mixed with 0.002 ml of reservoir solution containing 0.2 M potassium formate or potassium nitrate, pH 7.0-7.5, and 24-28% PEG 3350, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement	Leishmania major

Crystallization (Comment)

Organism

purified recombinant His-tagged catalytic core of methionyl-tRNA synthetase, containing a 3C protease cleavage site, in complex with the substrates MgATP2- and methionine, and in complex with two products, methionyladenylate and pyrophosphate, along with a Mg2+ ion that bridges them, hanging drop vapor diffusion, room temperature, 0.002 ml of 22 mg/ml protein in 25 mM HEPES, pH 7.0, 0.5 M NaCl, 0.025% sodium azide, 5% glycerol, 1 mM TCEP, 0.01 mM ZnCl2, 10 mM MgATP2- and 10 mM L-methionine, is mixed with 0.002 ml of reservoir solution containing 0.2 M potassium formate or potassium nitrate, pH 7.0-7.5, and 24-28% PEG 3350, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement

Leishmania major

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required. The Mg2+ ion has six atoms arranged in a square bipyramidal coordination shell and bridges the diphosphate and MetAMP products. It is interacting with two oxygen atoms from two different phosphoryl groups of the diphosphate, one oxygen from the MetAMP phosphoryl group, and three water molecules, though one of these is a bit distant for a typical Mg2+ ligand at approximately 2.5 A	Leishmania major
Zn2+	required	Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-methionine + tRNAMet	Leishmania major	-	AMP + diphosphate + L-methionyl-tRNAMet	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged catalytic core of MetRS from Escherichia coli using the expression vector AVA0421 by nickel affinity chromatography and gel filtration	Leishmania major

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-methionine + tRNAMet	-	Leishmania major	AMP + diphosphate + L-methionyl-tRNAMet	-	?
ATP + L-methionine + tRNAMet	binding structures of substrates and products, overview	Leishmania major	AMP + diphosphate + L-methionyl-tRNAMet	-	?

Subunits

Subunits	Comment	Organism
More	structure of LmMetRS in complex with two products, methionyladenylate and diphosphate, along with a Mg2+ ion that bridges them, the residues of the class I aaRS signature sequence motifs, KISKS and HIGH, make numerous contacts with the diphosphate, structure comparisons, overview	Leishmania major

Synonyms

Synonyms	Comment	Organism
methionyl-tRNA synthetase	-	Leishmania major
MetRS	-	Leishmania major
More	the enzyme belongs to the class I aminoacyl-tRNA synthetases	Leishmania major

Cofactor

Cofactor	Comment	Organism	Structure
ATP	as MgATP2-	Leishmania major