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Literature summary for 6.1.1.10 extracted from

  • Ingvarsson, H.; Jones, T.A.; Unge, T.
    Crystallization of Mycobacterium smegmatis methionyl-tRNA synthetase in the presence of methionine and adenosine (2009), Acta Crystallogr. Sect. F, 65, 618-620.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET101/D-TOPO, a His-Tag is attached, for expression in Escherichia coli BL21 StarDE3 cells Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of methionyl-tRNA synthetase is determined to a resolution of 2.1 A Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 determined by SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R3E2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose is used, in addition gel filtration on a HiLoad 16/60 Superdex-200 column is applied Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
MetRS
-
 Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycolicibacterium smegmatis