Cloned (Comment) | Organism |
---|---|
expression of His-tagged mutants in Escherichia coli strain XL-1 Blue | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
L13G | saturation mutagenesis, three mutant clones from screening of a saturation mutagenesis library, the mutants are capable of incorporating the long-chain amino acid azidonorleucine into recombinant proteins with modest efficiency | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + azidonorleucine + tRNAMet | Escherichia coli | activity of mutant L13G, overview | AMP + diphosphate + azidonorleucyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | Escherichia coli | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00959 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography | Escherichia coli |
recombinnat His-tagged mutants from Escherichia coli strain XL-1 Blue by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + azidonorleucine + tRNAMet | activity of mutant L13G, overview | Escherichia coli | AMP + diphosphate + azidonorleucyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MetRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |