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Literature summary for 6.1.1.10 extracted from

  • Kedar, G.C.; Brown-Driver, V.; Reyes, D.R.; Hilgers, M.T.; Stidham, M.A.; Shaw, K.J.; Finn, J.; Haselbeck, R.J.
    Evaluation of the metS and murB loci for antibiotic discovery using targeted antisense RNA expression analysis in Bacillus anthracis (2007), Antimicrob. Agents Chemother., 51, 1708-1718.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Application

Application Comment Organism
pharmacology the enzyme is an important antibiotic target Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
genes metS1 and metS2, library construction, subcloning in Escherichia coli strains DH5alpha and INV110 Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
additional information gene replacement mutagenesis, downregulation of metS1 by its antisense construct, xylose-induced antisense expression, antisense orientation is identified for the metS1 allele, while no such orientation bias is seen for the metS2 allele, attenuation of MetS1 enzyme expression hypersensitizes Bacillus anthracis cells to a MetS-specific antimicrobial compound Rx-000019, but not to other antibiotics that affect cell wall assembly, fatty acid biosynthesis, protein translation, or DNA replication, overview Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Bacillus anthracis essentiality of the metS1, nonessentiality of the metS2 for vegetative growth AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
 UM23C1-1and GRI strains, genes metS1 and metS2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Bacillus anthracis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet essentiality of the metS1, nonessentiality of the metS2 for vegetative growth Bacillus anthracis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Bacillus anthracis
MetS
-
 Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus anthracis