Cloned (Comment) | Organism |
---|---|
gene metE, genetic library construction and expression, expression of His-tagged mutant enzymes in strains DH10B and in DH10B Met- | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A256X | random mutagnesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
A256X | random mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
H301L | saturation mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
L13S | saturation mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M218A | random mutagnesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M218A | random mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M233I | random mutagnesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M233I | random mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M78L | random mutagnesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M78L | random mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M88F | random mutagnesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
M88F | random mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
additional information | high-throughput screening for mutant enzymes that enable residue-specific incorporation of noncanonical amino acids into the recombinant mutant enzymes in the bacterial cells, overview | Escherichia coli |
P257X | saturation mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
Y260L | saturation mutagenesis, the mutant shows altered amino acid substrate binding compared to the wild-type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Escherichia coli | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene metE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mutant enzymes from strain DH10B by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | residues L13, P257, Y260, and H301 are involved in the Met binding site | Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Escherichia coli |
MetRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |