Cloned (Comment) | Organism |
---|---|
modified gene metS, expression of M606 enzyme in Escherichia coli | Pyrococcus abyssi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant M606 enzyme, hanging drop vapor diffusion method, from 0.1 M MES, pH 6.5, 3,5% PEG 6000, and 2% dioxane, 22°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.9 A resolution | Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Pyrococcus abyssi | |
Mg2+ | - |
Pyrococcus abyssi | |
Zn2+ | two Zn2+ per protomer, binding site structures | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Pyrococcus abyssi | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9V011 | class I enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant M606 enzyme from Escherichia coli by ion exchange chromatography and gel filtration | Pyrococcus abyssi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Pyrococcus abyssi | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
ATP + L-methionine + tRNAMet | tRNA anticodon binding site structure | Pyrococcus abyssi | AMP + diphosphate + L-methionyl-tRNAMet | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure, structure of the dimerization domain | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Pyrococcus abyssi |
MetRS | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pyrococcus abyssi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | 60 | the enzyme is nearly inactive at 25°C, highest activity at 60°C | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Pyrococcus abyssi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pyrococcus abyssi |