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Literature summary for 6.1.1.10 extracted from
- A recurrent general RNA binding domain appended to plant methionyl-tRNA synthetase acts as a cis-acting cofactor for aminoacylation (2000), EMBO J., 19, 6908-6917.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination, overexpression of full-length wild-type and a deleted version lacking the C-terminal EMAPII-like domain in Escherichia coli BL21(DE3) | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00066 | - |
tRNAMet | in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme | Oryza sativa |  |
| 0.0007 | - |
tRNA fraction from Saccharomyces cerevisiae | pH 7.5, 25°C, wild-type enzyme | Oryza sativa | |
| 0.00075 | - |
tRNA fraction from Saccharomyces cerevisiae | pH 7.5, 25°C, truncated mutant | Oryza sativa | |
| 0.0065 | - |
tRNAMet | in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Oryza sativa | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
truncated enzyme, gel filtration | Oryza sativa |
| 66500 | - |
1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE | Oryza sativa |
| 89700 | - |
1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE | Oryza sativa |
| 110000 | - |
wild-type enzyme, gel filtration | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Oryza sativa | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q9ZTS1 | enzyme contains a supplementary C-terminal domain related to endothelial monocyte activating polypeptide II, i.e. EMAPII, a cytokine | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli | Oryza sativa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet | the amino acid acceptor arm of tRNAMet binds to the plant enzyme | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNA fraction from Saccharomyces cerevisiae | - |
Oryza sativa | ? | - |
? | |
| ATP + L-methionine + tRNAAsp | only the wild-type enzyme, not the C-terminally deleted enzyme mutant | Oryza sativa | AMP + diphosphate + L-methionyl-tRNAAsp | - |
? | |
| ATP + L-methionine + tRNAMet | - |
Oryza sativa | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | the EMAPII-like C-terminal appendix domain provides the enzyme with non-specific tRNA binding properties, the deleted enzyme form missing this domain is therefore more specific and shows a 10fold lower Km for the tRNA substrate, enzyme is also active with tRNAMet from Saccharomyces cerevisiae | Oryza sativa | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE | Oryza sativa |
| More | at high protein concentration the enzyme can form a dimer in vitro | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methionine translase | - |
Oryza sativa |
| Methionine--tRNA ligase | - |
Oryza sativa |
| Methionyl tRNA synthetase | - |
Oryza sativa |
| Methionyl-transfer ribonucleate synthetase | - |
Oryza sativa |
| Methionyl-transfer ribonucleic acid synthetase | - |
Oryza sativa |
| Methionyl-transfer RNA synthetase | - |
Oryza sativa |
| MetRS | - |
Oryza sativa |
| Synthetase, methionyl-transfer ribonucleate | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
tRNA fraction from Saccharomyces cerevisiae | pH 7.5, 25°C, truncated mutant | Oryza sativa | |
| 0.29 | - |
tRNAMet | in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme | Oryza sativa | |
| 0.36 | - |
tRNAMet | in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant | Oryza sativa | |
| 0.53 | - |
tRNA fraction from Saccharomyces cerevisiae | pH 7.5, 25°C, wild-type enzyme | Oryza sativa | |
| 6.08 | - |
tRNA fraction from Saccharomyces cerevisiae | pH 7.5, 25°C, wild-type enzyme | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Oryza sativa | |
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Release 2023.1 (January 2023)
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