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Literature summary for 6.1.1.10 extracted from

  • Armstrong, J.B.; Fairfield, J.A.
    A new method for the isolation of methionyl transfer synthetase mutants from Escherichia coli (1975), Can. J. Microbiol., 21, 754-758.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information mutant strains with an increased Km for methionine Escherichia coli

General Stability

General Stability Organism
several cycles of freezing and thawing, cause no loss of activity of wild-type enzyme in crude extract Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
Met wild-type enzyme Escherichia coli
0.48 1.63 Met Km-values of various mutant strains Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
and mutant strains with an increased Km for methionine
-

Storage Stability

Storage Stability Organism
-20°C, about 35% loss of activity after 6 months, wild-type enzyme in crude extract Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
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