Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strains with an increased Km for methionine | Escherichia coli |
General Stability | Organism |
---|---|
several cycles of freezing and thawing, cause no loss of activity of wild-type enzyme in crude extract | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
Met | wild-type enzyme | Escherichia coli | |
0.48 | 1.63 | Met | Km-values of various mutant strains | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
and mutant strains with an increased Km for methionine | - |
Storage Stability | Organism |
---|---|
-20°C, about 35% loss of activity after 6 months, wild-type enzyme in crude extract | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |