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Literature summary for 6.1.1.1 extracted from

  • Iraha, F.; Oki, K.; Kobayashi, T.; Ohno, S.; Yokogawa, T.; Nishikawa, K.; Yokoyama, S.; Sakamoto, K.
    Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion (2010), Nucleic Acids Res., 38, 3682-3691.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.1

Application

Application Comment Organism
biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Saccharomyces cerevisiae
biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Methanocaldococcus jannaschii
biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
into the vectors pACYC184 and pAp102 Saccharomyces cerevisiae
into the vectors pACYC184 and pAp102 Methanocaldococcus jannaschii
into the vectors pACYC184 and pAp102 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the iodoTyrRS-ec-3-azide-L-tyrosine structure is determined at a resolution of 1.8 A Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information the Escherichia coli TyrRS-tRNATyr pair is functionally replaced by the Methanocaldococcus jannaschii tyrosine pair Methanocaldococcus jannaschii
additional information the Escherichia coli TyrRS-tRNATyr pair is functionally replaced by the Saccharomyces cerevisiae tyrosine pair Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr Escherichia coli
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AGJ9
-
-
Methanocaldococcus jannaschii
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-azido-L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
ATP + 3-azido-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
ATP + 3-azido-L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
 Saccharomyces cerevisiae
Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
Tyrosyl-tRNA synthetase
-
 Escherichia coli
TyrRS
-
 Saccharomyces cerevisiae
TyrRS
-
 Methanocaldococcus jannaschii
TyrRS
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae
ATP
-
 Methanocaldococcus jannaschii
ATP
-
 Escherichia coli