Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | inhibition is weakened by chloride | Escherichia coli | |
AMP | inhibition is weakened by chloride | Geobacillus stearothermophilus | |
chloride | inhibition in presence of 1 mM free Mg2+, no inhibition in presence of 10 mM free Mg2+ | Escherichia coli | |
chloride | inhibition in presence of 1 mM free Mg2+, no inhibition in presence of 10 mM free Mg2+ | Geobacillus stearothermophilus | |
diphosphate | inhibition is strengthened by chloride | Escherichia coli | |
diphosphate | inhibition is strengthened by chloride | Geobacillus stearothermophilus | |
additional information | no inhibition by acetate up to 200 mM in presence of 1 mM free Mg2+ | Escherichia coli | |
sulfate | inhibition in presence of 1 mM free Mg2+ | Escherichia coli | |
sulfate | inhibition in presence of 1 mM free Mg2+ | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
additional information | - |
additional information | kinetics | Geobacillus stearothermophilus | |
0.000022 | - |
tRNATyr | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
0.000037 | - |
tRNATyr | in presence of 50 mM KCl, pH 7.4, 30°C | Escherichia coli | |
0.000093 | - |
tRNATyr | in presence of 100 mM KCl, pH 7.4, 30°C | Escherichia coli | |
0.00024 | - |
tRNATyr | in presence of 150 mM KCl, pH 7.4, 30°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, shows regulatory function | Geobacillus stearothermophilus | |
Mg2+ | required, shows regulatory function, binding of 1 Mg2+ per tRNA molecule for the transfer reaction, binding to the tRNA is weakened by chloride | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | Escherichia coli | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? | |
ATP + tyrosine + tRNATyr | Geobacillus stearothermophilus | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
C6, purified enzyme | - |
Geobacillus stearothermophilus | - |
strain ATCC 12980 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr | mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + tyrosine + tRNATyr | - |
Escherichia coli | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
ATP + tyrosine + tRNATyr | - |
Geobacillus stearothermophilus | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
ATP + tyrosine + tRNATyr | tRNATyr substrate from Escherichia coli | Geobacillus stearothermophilus | AMP + Tyr-tRNATyr + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosine tRNA synthetase | - |
Escherichia coli |
Tyrosyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
TyrRS | - |
Escherichia coli |
TyrRS | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
7.4 | - |
assay at | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
additional information | - |
additional information | inhibition kinetics | Geobacillus stearothermophilus | |
0.048 | - |
diphosphate | in presence of 150 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.056 | - |
diphosphate | in presence of 0.9 mM KCl, pH 7.4, 30°C | Escherichia coli | |
0.1 | - |
diphosphate | in presence of 50 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.12 | - |
diphosphate | in presence of 0.5 mM KCl, pH 7.4, 30°C | Escherichia coli | |
0.14 | - |
diphosphate | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
0.23 | - |
diphosphate | in absence of KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.32 | - |
AMP | in absence of KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.35 | - |
AMP | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
0.5 | - |
AMP | in presence of 0.5 mM KCl, pH 7.4, 30°C | Escherichia coli | |
0.5 | - |
AMP | in presence of 50 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
0.9 | - |
AMP | in presence of 0.9 mM KCl, pH 7.4, 30°C | Escherichia coli | |
2.15 | - |
AMP | in presence of 150 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus |