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Literature summary for 6.1.1.1 extracted from

  • Buonocore, V.; Harris, M.H.; Schlesinger, S.
    Properties of tyrosyl transfer ribonucleic acid synthetase from two tyrS mutants of Escherichia coli K-12 (1972), J. Biol. Chem., 247, 843-4849.
No PubMed abstract available
Search for more literature for 6.1.1.1

General Stability

General Stability Organism
Tyrosine and/or ATP stabilize Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
tRNATyr
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information values for mutant strains Escherichia coli
0.0002
-
 L-tyrosyl-tRNATyr deacylation Escherichia coli
0.00052
-
 tRNATyr acylation Escherichia coli
0.008
-
 tyrosine acylation Escherichia coli
0.012
-
 tyrosine ATP-diphosphate exchange reaction Escherichia coli
0.5
-
 ATP ATP-diphosphate exchange reaction Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Escherichia coli
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 sucrose density gradient centrifugation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Storage Stability

Storage Stability Organism
4°C, 20 mM Tris-HCl buffer, pH 7, 80% loss of activity within 48 h, 5% loss of activity in presence of 10% glycerol and 3 mM 2-mercaptoethanol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + diphosphate
-
 Escherichia coli ATP
-
 ?
AMP + diphosphate + L-tyrosyl-tRNATyr
-
 Escherichia coli ATP + L-tyrosine + tRNATyr
-
 ?
ATP + tyrosine + tRNATyr r Escherichia coli AMP + Tyr-tRNATyr + diphosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46
-
 40% loss of activity in 4-12 min, depending on the bacterial strains used for purification, stabilization by substrates Escherichia coli