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Literature summary for 5.4.99.B4 extracted from

  • Ero, R.; Leppik, M.; Liiv, A.; Remme, J.
    Specificity and kinetics of 23S rRNA modification enzymes RlmH and RluD (2010), RNA, 16, 2075-2084.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00098
-
50S ribosomal subunit His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine RluD isomerizes all three uridines (U1911, U1915, and U1917) in the stem–loop 69 of 23S rRNA to pseudouridines Escherichia coli 23S rRNA pseudouridine
-
?
50S ribosomal subunit
-
Escherichia coli ?
-
?
50S rRNA uridine
-
Escherichia coli 50S rRNA pseudouridine
-
?

Synonyms

Synonyms Comment Organism
pseudouridine synthase
-
Escherichia coli
RluD
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.033
-
50S ribosomal subunit His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
33.5
-
50S ribosomal subunit His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C Escherichia coli