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Literature summary for 5.4.99.5 extracted from

  • Okvist, M.; Sasso, S.; Roderer, K.; Kast, P.; Krengel, U.
    A novel noncovalent complex of chorismate mutase and DAHP synthase from Mycobacterium tuberculosis: protein purification, crystallization and X-ray diffraction analysis (2009), Acta Crystallogr. Sect. F, 65, 1048-1052.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase
-
Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain KA13 Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
alone and in complex with 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, hanging drop vapor diffusion method, using 25% (w/v) PEG 1500 and 0.1 M MMT buffer (L-malic acid, MES, Tris) pH 8.0-9.0 Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WIC1
-
-
Mycobacterium tuberculosis H37Rv P9WIC1
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA agarose column chromatography, and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate Mycobacterium tuberculosis Prephenate
-
?
Chorismate the enzyme catalyzes the Claisen rearrangement of chorismate to prephenate Mycobacterium tuberculosis H37Rv Prephenate
-
?

Synonyms

Synonyms Comment Organism
Rv0948c
-
Mycobacterium tuberculosis