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Literature summary for 5.4.99.5 extracted from

  • Lim, S.; Springstead, J.R.; Yu, M.; Bartkowski, W.; Schroeder, I.; Monbouquette, H.G.
    Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus (2009), Extremophiles, 13, 191-198.
    View publication on PubMed
Search for more literature for 5.4.99.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
additional information activity is not affected by both amino acids Phe and Tyr at concentrations up to 0.5 mM Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information due to instability of chorismate at higher temperature, a Km value is not determined Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 6 * 66000, SDS-PAGE Archaeoglobus fulgidus
70946
-
 6 * 70946, calculated from sequence Archaeoglobus fulgidus
420000
-
 gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Archaeoglobus fulgidus
-
 Prephenate
-
 ?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30012
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Archaeoglobus fulgidus Prephenate
-
 ?
Chorismate the enzyme is involved in aromatic amino acid biosynthesis Archaeoglobus fulgidus Prephenate
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 66000, SDS-PAGE Archaeoglobus fulgidus
homohexamer 6 * 70946, calculated from sequence Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
AroQ gene name. Trifunctional enzyme EC 5.4.99.5 (chorismate mutase)/EC 4.2.1.51 (prephenate dehydratase)/EC 1.3.1.12 (prephenate dehydrogenase) Archaeoglobus fulgidus
CM/PDT/PDHG trifunctional enzyme EC 5.4.99.5 (chorismate mutase)/EC 4.2.1.51 (prephenate dehydratase)/EC 1.3.1.12 (prephenate dehydrogenase) Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Archaeoglobus fulgidus

General Information

General Information Comment Organism
metabolism the enzyme is involved in aromatic amino acid biosynthesis Archaeoglobus fulgidus