Any feedback?
Literature summary for 5.4.99.5 extracted from
- HARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon methanol utilization (2000), J. Bacteriol., 182, 4188-4197.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| tryptophan | heterotrophic positive effector | Ogataea angusta |  |
| tryptophan | heterotrophic positive effector | Saccharomyces cerevisiae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in a Saccharomyces cerevisiae aro7DELTA strain | Ogataea angusta |
| overexpression in Hansenula polymorpha | Ogataea angusta |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tyrosine | heterotrophic feedback inhibitor | Ogataea angusta | |
| tyrosine | heterotrophic feedback inhibitor | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | sigmoid substrate saturation curve with S0.5: 16.7 mM for chorismate at 37°C and S0.5: 12 mM for chorismate at 37°C in presence of 0.1 mM tyrosine | Ogataea angusta | |
| 1.6 | - |
chorismate | 37°C, in presence of 0.01 mM tryptophan | Ogataea angusta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
32000, SDS-PAGE | Ogataea angusta |
| 70000 | - |
PAGE | Ogataea angusta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | Ogataea angusta | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
| Chorismate | Saccharomyces cerevisiae | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | Q9P4D8 | - |
- |
| Saccharomyces cerevisiae | P32178 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 168 | - |
Superdex 200pg pool | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Chorismate | - |
Ogataea angusta | Prephenate | - |
? | |
| Chorismate | - |
Saccharomyces cerevisiae | Prephenate | - |
? | |
| Chorismate | biosynthesis of aromatic amino acids | Ogataea angusta | Prephenate | - |
? | |
| Chorismate | biosynthesis of aromatic amino acids | Saccharomyces cerevisiae | Prephenate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 32000, SDS-PAGE | Ogataea angusta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chorismate mutase | - |
Ogataea angusta |
| chorismate mutase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
decrease in turnover at temperatures higher than 38°C | Saccharomyces cerevisiae |
| 48 | - |
maximum enzymatic activity | Ogataea angusta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.8 | - |
chorismate | 37°C, in presence of 0.1 mM tyrosine | Ogataea angusta | |
| 89.3 | - |
chorismate | 37°C, in presence of 0.1 mM tyrosine | Ogataea angusta | |
| 367 | - |
chorismate | 37°C | Ogataea angusta | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
