Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.5 extracted from

  • Gamper, M.; Hilvert, D.; Kast, P.
    Probing the role of the C-terminus of Bacillus subtilis chorismate mutase by a novel random protein-termination strategy (2000), Biochemistry, 39, 14087-14094.
    View publication on PubMed
Search for more literature for 5.4.99.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
DELTA117-127 large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
DELTA118-127 large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
DELTA118-127/K111N/A112S/V113N large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
DELTA118-127/R116L/P117T large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
DELTA119-127/D118N large increase in KM and slower turnover relative to wild-type enzyme Bacillus subtilis
R90Q complete inactivation of enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
 chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate Bacillus subtilis
3.9
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate Bacillus subtilis
4
-
 chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate Bacillus subtilis
4.1
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate Bacillus subtilis
9.3
-
 chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate Bacillus subtilis
15
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate Bacillus subtilis
16
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/R116L/P117T, with 3.9 mM substrate Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Bacillus subtilis biosynthesis of aromatic amino acids Prephenate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
 Bacillus subtilis Prephenate
-
 ?
Chorismate biosynthesis of aromatic amino acids Bacillus subtilis Prephenate
-
 ?

Synonyms

Synonyms Comment Organism
BsCM
-
 Bacillus subtilis
chorismate mutase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 5.8 mM substrate Bacillus subtilis
23
-
 chorismate 30°C, pH 7.5, mutant DELTA119-127/D118N, with 5.8 mM substrate Bacillus subtilis
24
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127, with 3.4 mM substrate Bacillus subtilis
26
-
 chorismate 30°C, pH 7.5, mutant DELTA117-127, with 3.8 mM substrate and DELTA 118-127/R116L/P117T, with 3.9 mM substrate Bacillus subtilis
30
-
 chorismate 30°C, pH 7.5, mutant DELTA118-127/K111N/A112S/V113N, with 4 mM substrate Bacillus subtilis
47
-
 chorismate 30°C, pH 7.5, wild-type, with 4 mM substrate Bacillus subtilis