Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.5 extracted from

  • Christendat, D.; Saridakis, V.C.; Turnbull, J.L.
    Use of site-directed mutagenesis to identify residues specific for each reaction catalyzed by chorismate mutase-prephenate dehydrogenase from Escherichia coli (1998), Biochemistry, 37, 15703-15712.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
H131A 30% activity compared to wild-type enzyme Escherichia coli
H153N lower turnover and higher KM than wild-type enzyme Escherichia coli
H189N much lower turnover than wild-type enzyme Escherichia coli
H197N lower turnover and higher KM than wild-type enzyme Escherichia coli
H239N lower turnover and higher KM than wild-type enzyme Escherichia coli
H245N lower turnover and higher KM than wild-type enzyme Escherichia coli
H257A lower turnover and higher KM than wild-type enzyme Escherichia coli
H265A lower turnover and higher KM than wild-type enzyme Escherichia coli
H347N lower turnover than wild-type enzyme Escherichia coli
K37A more poorly expressed than wild-type, inactive and instable Escherichia coli
K37Q no activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
prephenate competitive inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
chorismate 30°C, pH 7.2, mutant H347N Escherichia coli
0.045
-
chorismate 30°C, pH 7.2, wild-type Escherichia coli
0.051
-
chorismate 30°C, pH 7.2, mutant H153N Escherichia coli
0.053
-
chorismate 30°C, pH 7.2, mutant H131A Escherichia coli
0.068
-
chorismate 30°C, pH 7.2, mutant H197N Escherichia coli
0.098
-
chorismate 30°C, pH 7.2, mutant H257A Escherichia coli
0.099
-
chorismate 30°C, pH 7.2, mutant H265A Escherichia coli
0.126
-
chorismate 30°C, pH 7.2, mutant H239N Escherichia coli
0.225
-
chorismate 30°C, pH 7.2, mutant H245N Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Chorismate Escherichia coli biosynthesis of aromatic amino acids Prephenate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chorismate
-
Escherichia coli Prephenate
-
?
Chorismate biosynthesis of aromatic amino acids Escherichia coli Prephenate
-
?

Synonyms

Synonyms Comment Organism
chorismate mutase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information turnover of mutant H189N enzyme is lower than 0.0025 per second Escherichia coli
additional information
-
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide turnover of mutant H189N enzyme is lower than 0.0025 per second Escherichia coli
2.3
-
chorismate 30°C, pH 7.2, mutant H239N Escherichia coli
4.8
-
chorismate 30°C, pH 7.2, mutant H245N Escherichia coli
6
-
chorismate 30°C, pH 7.2, mutant H347N Escherichia coli
7.2
-
chorismate 30°C, pH 7.2, mutant H131A Escherichia coli
8
-
chorismate 30°C, pH 7.2, mutant H257A Escherichia coli
10
-
chorismate 30°C, pH 7.2, mutant H153N Escherichia coli
15
-
chorismate 30°C, pH 7.2, mutant H265A Escherichia coli
16
-
chorismate 30°C, pH 7.2, mutant H197N Escherichia coli
27
-
chorismate 30°C, pH 7.2, wild-type Escherichia coli