Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H131A | 30% activity compared to wild-type enzyme | Escherichia coli |
H153N | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H189N | much lower turnover than wild-type enzyme | Escherichia coli |
H197N | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H239N | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H245N | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H257A | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H265A | lower turnover and higher KM than wild-type enzyme | Escherichia coli |
H347N | lower turnover than wild-type enzyme | Escherichia coli |
K37A | more poorly expressed than wild-type, inactive and instable | Escherichia coli |
K37Q | no activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
prephenate | competitive inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
chorismate | 30°C, pH 7.2, mutant H347N | Escherichia coli | |
0.045 | - |
chorismate | 30°C, pH 7.2, wild-type | Escherichia coli | |
0.051 | - |
chorismate | 30°C, pH 7.2, mutant H153N | Escherichia coli | |
0.053 | - |
chorismate | 30°C, pH 7.2, mutant H131A | Escherichia coli | |
0.068 | - |
chorismate | 30°C, pH 7.2, mutant H197N | Escherichia coli | |
0.098 | - |
chorismate | 30°C, pH 7.2, mutant H257A | Escherichia coli | |
0.099 | - |
chorismate | 30°C, pH 7.2, mutant H265A | Escherichia coli | |
0.126 | - |
chorismate | 30°C, pH 7.2, mutant H239N | Escherichia coli | |
0.225 | - |
chorismate | 30°C, pH 7.2, mutant H245N | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | Escherichia coli | biosynthesis of aromatic amino acids | Prephenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
Chorismate | biosynthesis of aromatic amino acids | Escherichia coli | Prephenate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover of mutant H189N enzyme is lower than 0.0025 per second | Escherichia coli | |
additional information | - |
2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide | turnover of mutant H189N enzyme is lower than 0.0025 per second | Escherichia coli | |
2.3 | - |
chorismate | 30°C, pH 7.2, mutant H239N | Escherichia coli | |
4.8 | - |
chorismate | 30°C, pH 7.2, mutant H245N | Escherichia coli | |
6 | - |
chorismate | 30°C, pH 7.2, mutant H347N | Escherichia coli | |
7.2 | - |
chorismate | 30°C, pH 7.2, mutant H131A | Escherichia coli | |
8 | - |
chorismate | 30°C, pH 7.2, mutant H257A | Escherichia coli | |
10 | - |
chorismate | 30°C, pH 7.2, mutant H153N | Escherichia coli | |
15 | - |
chorismate | 30°C, pH 7.2, mutant H265A | Escherichia coli | |
16 | - |
chorismate | 30°C, pH 7.2, mutant H197N | Escherichia coli | |
27 | - |
chorismate | 30°C, pH 7.2, wild-type | Escherichia coli |