Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-Trp | enzyme form CM1 is activated | Ruta graveolens | |
L-Trp | enzyme form CM2 is unaffected | Ruta graveolens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Methyl-DL-Trp | enzyme form CM1 is inhibited, enzyme form CM2 not | Ruta graveolens | |
6-Methyl-DL-Trp | enzyme form CM1 is inhibited, enzyme form CM2 not | Ruta graveolens | |
Ba2+ | - |
Ruta graveolens | |
Co2+ | - |
Ruta graveolens | |
Cu2+ | strong | Ruta graveolens | |
DL-3-fluoro-Phe | enzyme form CM1 is inhibited, enzyme form CM2 not | Ruta graveolens | |
DL-5-Fluoro-Trp | enzyme form CM1 is inhibited, enzyme form CM2 not | Ruta graveolens | |
DL-5-hydroxy-Trp | enzyme form CM1 is inhibited, enzyme form CM2 not | Ruta graveolens | |
Fe2+ | - |
Ruta graveolens | |
Hg2+ | HgCl2 | Ruta graveolens | |
L-Phe | - |
Escherichia coli | |
L-Phe | enzyme form CM1 is inhibited; Trp reverses inhibition | Ruta graveolens | |
L-Tyr | - |
Escherichia coli | |
L-Tyr | enzyme form CM1 is inhibited; enzyme form CM2 is not inhibited; Trp reverses inhibition | Ruta graveolens | |
Mn2+ | - |
Ruta graveolens | |
Pb2+ | - |
Ruta graveolens | |
PCMB | - |
Ruta graveolens | |
Zn2+ | strong | Ruta graveolens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
chorismate | enzyme form CM2 | Ruta graveolens | |
1.1 | - |
chorismate | enzyme CM1 | Ruta graveolens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
enzyme form CM2, gel filtration | Ruta graveolens |
56000 | - |
enzyme form CM1, gel filtration | Ruta graveolens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Ruta graveolens | - |
2 isoenzymes: CM1 and CM2 | - |
Purification (Comment) | Organism |
---|---|
partial | Ruta graveolens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Ruta graveolens | - |
Storage Stability | Organism |
---|---|
-20°C, enzyme form CM1 and CM2 are stable | Ruta graveolens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
Chorismate | - |
Ruta graveolens | Prephenate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 37 | enzyme form CM1 | Ruta graveolens |
56 | - |
enzyme form CM2 | Ruta graveolens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
5 min, enzyme form CM1, 50% loss of activity | Ruta graveolens |
50 | 60 | 5 min, enzyme form CM2, stable | Ruta graveolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | enzyme form CM2 | Ruta graveolens |
7.8 | - |
enzyme form CM1 | Ruta graveolens |