Protein Variants | Comment | Organism |
---|---|---|
K19M | kcat is 8fold lower than wild-type value. KM-value is 11fold higher than wild-type value | Escherichia coli |
K19R | kcat is 10fold lower than wild-type value. KM-value is 6fold higher than wild-type value | Escherichia coli |
additional information | mutant enzymes with substitution of the nearly invariant lysine and proline residues in motif I of RluA display only very mild kinetic impairment. Substitution of the aligned lysine and proline residues reduces structural stability | Escherichia coli |
P20G | kcat is 4.8fold lower than wild-type value. KM-value is 2.2fold higher than wild-type value | Escherichia coli |
P20L | kcat is 2.1fold lower than wild-type value. KM-value is 3.5fold higher than wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000148 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
0.000324 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
0.000517 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli | |
0.0009 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
0.00161 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60340 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine55 | substrate: Escherichia coli tRNAPhe | Escherichia coli | tRNA pseudouridine552 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TruB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
36 | - |
Tm-value for mutant enzyme P20L is 35.9°C | Escherichia coli |
38 | - |
Tm-value for mutant enzyme P20G is 38.3°C | Escherichia coli |
40 | - |
Tm-value for wild-type enzyme is 40.2°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
0.012 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli | |
0.11 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
0.23 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
0.52 | - |
tRNA uridine55 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.43 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
7.45 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19M | Escherichia coli | |
12.2 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme K19R | Escherichia coli | |
709.8 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20G | Escherichia coli | |
1006 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P20L | Escherichia coli |