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Literature summary for 5.4.99.25 extracted from

  • Pan, H.; Agarwalla, S.; Moustakas, D.T.; Finer-Moore, J.; Stroud, R.M.
    Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit (2003), Proc. Natl. Acad. Sci. USA, 100, 12648-12653.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
vapor-phase diffusion method, crystal structure of TruB apoenzyme in the absence of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound forms of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity Escherichia coli
vapor-phase diffusion method, crystal structure of TruB in complex with 17-base stem-loop of RNA. Comparison of the TruB apoenzyme from Escherichia coli and the RNA-bound form of Thermotoga maritima TruB provides insight into the structural basis for RNA recognition and specificity Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Escherichia coli P60340
-
-
Thermotoga maritima Q9WZW0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Thermotoga maritima

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine55 = tRNA pseudouridine55 this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit Escherichia coli
a
tRNA uridine55 = tRNA pseudouridine55 this enzyme undergoes significant conformational changes on binding to its substrate. These conformational changes include the ordering of the thumb loop, which binds right into the RNA hairpin loop, and a 10° hinge movement of the C-terminal domain. TruB recognizes its RNA substrate through a combination of rigid docking and induced fit, with TruB first rigidly binding to its target and then maximizing the interaction by induced fit Thermotoga maritima
a

Synonyms

Synonyms Comment Organism
RNA pseudouridine synthase TruB
-
 Escherichia coli
RNA pseudouridine synthase TruB
-
 Thermotoga maritima
TruB
-
 Escherichia coli
TruB
-
 Thermotoga maritima