Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C174A | kcat is 1.3fold higher than wild-type value, KM is 1.1fold lower than wild-type value | Escherichia coli |
C193V | kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value | Escherichia coli |
C58A | kcat is 2.2fold higher than wild-type value, KM is 1.2fold higher than wild-type value | Escherichia coli |
C58A/C174A/C193A | kcat is 1.1fold lower than wild-type value, KM is 1.2fold lower than wild-type value | Escherichia coli |
additional information | substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000124 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A | Escherichia coli | |
0.000127 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A | Escherichia coli | |
0.000146 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
0.000177 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A | Escherichia coli | |
0.000218 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P60340 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
tRNA uridine55 = tRNA pseudouridine55 | RluA does not require thiol(ate) groups to effect catalysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine55 | substrate: Escherichia coli tRNAPhe | Escherichia coli | tRNA pseudouridine55 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TruB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A | Escherichia coli | |
0.12 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
0.16 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A | Escherichia coli | |
0.18 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V | Escherichia coli | |
0.26 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
821.9 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme | Escherichia coli | |
825 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V | Escherichia coli | |
887 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A | Escherichia coli | |
1260 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A | Escherichia coli | |
1469 | - |
tRNA uridine55 | pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A | Escherichia coli |