Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.25 extracted from

  • Ramamurthy, V.; Swann, S.; Spedaliere, C.; Mueller, E.
    Role of cysteine residues in pseudouridine synhases of different families (1999), Biochemistry, 38, 13106-13111.
    View publication on PubMed
Search for more literature for 5.4.99.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
C174A kcat is 1.3fold higher than wild-type value, KM is 1.1fold lower than wild-type value Escherichia coli
C193V kcat is 1.5fold higher than wild-type value, KM is 1.5fold higher than wild-type value Escherichia coli
C58A kcat is 2.2fold higher than wild-type value, KM is 1.2fold higher than wild-type value Escherichia coli
C58A/C174A/C193A kcat is 1.1fold lower than wild-type value, KM is 1.2fold lower than wild-type value Escherichia coli
additional information substitution of cysteine for amino acids with nonnucleophilic side chains does not significantly alter the catalytic activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000124
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A Escherichia coli
0.000127
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A Escherichia coli
0.000146
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
0.000177
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A Escherichia coli
0.000218
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P60340
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
tRNA uridine55 = tRNA pseudouridine55 RluA does not require thiol(ate) groups to effect catalysis Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tRNA uridine55 substrate: Escherichia coli tRNAPhe Escherichia coli tRNA pseudouridine55
-
 ?

Synonyms

Synonyms Comment Organism
TruB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A Escherichia coli
0.12
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
0.16
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A Escherichia coli
0.18
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V Escherichia coli
0.26
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
821.9
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe. wild-type enzyme Escherichia coli
825
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C193V Escherichia coli
887
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A/C174A/C193A Escherichia coli
1260
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C174A Escherichia coli
1469
-
 tRNA uridine55 pH 7.5, 37°C, substrate: Escherichia coli tRNAPhe, mutant enzyme C58A Escherichia coli