Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.23 extracted from

  • Leppik, M.; Peil, L.; Kipper, K.; Liiv, A.; Remme, J.
    Substrate specificity of the pseudouridine synthase RluD in Escherichia coli (2007), FEBS J., 274, 5759-5766.
    View publication on PubMed
Search for more literature for 5.4.99.23

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917 Escherichia coli pseudouridine synthase RluD converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA. RluD is the only pseudouridine synthase that is required for normal growth in Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23S rRNA uridine1911/uridine1915/uridine1917 pseudouridine synthase RluD converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA. RluD is the only pseudouridine synthase that is required for normal growth in Escherichia coli Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?
23S rRNA uridine1911/uridine1915/uridine1917 pseudouridine synthase RluD converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA. RluD isomerizes uridine in vivo only at positions 1911, 1915, and 1917, regardless of the presence of uridine at other positions in the loop of helix 69 of 23S rRNA variants. Substitution of one uridine by cytosine has no effect on the conversion of others (i.e. formation of pseudouridines at positions 1911, 1915, and 1917 are independent of each other). The substrate specificity of the pseudouridine synthase RluD is analyzed in vivo by using 23S rRNA variants. A1916 is the only position in the loop of helix 69, where mutations affect the RluD specific pseudouridine formation Escherichia coli 23S rRNA pseudouridine1911/pseudouridine1915/pseudouridine1917
-
 ?

Synonyms

Synonyms Comment Organism
pseudouridine synthase RluD
-
 Escherichia coli
RluD
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function pseudouridine synthase RluD converts uridines at positions 1911, 1915, and 1917 of 23S rRNA to pseudouridines. These nucleotides are located in the functionally important helix-loop 69 of 23S rRNA. RluD is the only pseudouridine synthase that is required for normal growth in Escherichia coli. RluD directed isomerization of uridines occurs as a late step during the assembly of the (50S) large ribosomal subunit Escherichia coli