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Literature summary for 5.4.99.22 extracted from
- The mechanism of pseudouridine synthases from a covalent complex with RNA, and alternate specificity for U2605 versus U2604 between close homologs (2014), Nucleic Acids Res., 42, 2037-2048.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the 1.3 A structure of RluB in complex with a 21-mer stem-loop substrate, in which U2605 is substituted by 5-fluorouridine. The structure reveals a covalent bond between the phenolic hydroxyl of the conserved active site Tyr140 and C6 of the isomerized 5-fluorouridine | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P37765 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RluB | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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