Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.2 extracted from

  • Han, Y.; Hawkins, A.S.; Adams, M.W.; Kelly, R.M.
    Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle (2012), Appl. Environ. Microbiol., 78, 6194-6202.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 2 * 17000 + 2 * 70000, SDS-PAGE Metallosphaera sedula
70000
-
 2 * 17000 + 2 * 70000, SDS-PAGE Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-methylmalonyl-CoA Metallosphaera sedula the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway succinyl-CoA
-
 r
(R)-methylmalonyl-CoA Metallosphaera sedula DSM 5348 the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway succinyl-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YEG1 and A4YIE3 A4YEG1: catalytic subunit of methylmalonyl-CoA mutase, A4YIE3: coenzyme B12-binding subunit of methylmalonyl-CoA mutase
-
Metallosphaera sedula DSM 5348 A4YEG1 and A4YIE3 A4YEG1: catalytic subunit of methylmalonyl-CoA mutase, A4YIE3: coenzyme B12-binding subunit of methylmalonyl-CoA mutase
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metallosphaera sedula

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
 75°C, pH not specified in the publication Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-methylmalonyl-CoA
-
 Metallosphaera sedula succinyl-CoA
-
 r
(R)-methylmalonyl-CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula succinyl-CoA
-
 r
(R)-methylmalonyl-CoA
-
 Metallosphaera sedula DSM 5348 succinyl-CoA
-
 r
(R)-methylmalonyl-CoA the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula DSM 5348 succinyl-CoA
-
 r

Subunits

Subunits Comment Organism
heterotetramer 2 * 17000 + 2 * 70000, SDS-PAGE Metallosphaera sedula

Synonyms

Synonyms Comment Organism
MCM
-
 Metallosphaera sedula
Msed_0638 locus name, catalytic subunit of methylmalonyl-CoA mutase Metallosphaera sedula
Msed_2055 locus name, coenzyme B12-binding subunit of methylmalonyl-CoA mutase Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Metallosphaera sedula

Expression

Organism Comment Expression
Metallosphaera sedula expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation up

General Information

General Information Comment Organism
physiological function the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway Metallosphaera sedula