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Literature summary for 5.4.99.2 extracted from
- Importance of the histidine ligand to coenzyme B12 in the reaction catalyzed by methylmalonyl-CoA mutase (2002), J. Biol. Chem., 277, 18523-18527.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H610A | weakened affinity to the cofactor and much lower turnover than wild-type enzyme | Propionibacterium freudenreichii subsp. shermanii |
| H610N | weakened affinity to the cofactor and much lower turnover than wild-type enzyme | Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00024 | - |
adenosylcobalamin | 37°C, wild-type | Propionibacterium freudenreichii subsp. shermanii |  |
| 0.00045 | - |
adenosylcobalamin | 37°C, mutant H610A | Propionibacterium freudenreichii subsp. shermanii | |
| 0.00052 | - |
adenosylcobalamin | 37°C, mutant H610N | Propionibacterium freudenreichii subsp. shermanii | |
| 0.102 | - |
(R)-2-methylmalonyl-CoA | 37°C, mutant H610N | Propionibacterium freudenreichii subsp. shermanii | |
| 0.133 | - |
(R)-2-methylmalonyl-CoA | 37°C, wild-type | Propionibacterium freudenreichii subsp. shermanii | |
| 0.364 | - |
(R)-2-methylmalonyl-CoA | 37°C, mutant H610A | Propionibacterium freudenreichii subsp. shermanii | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-methylmalonyl-CoA | Propionibacterium freudenreichii subsp. shermanii | - |
succinyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-methylmalonyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | succinyl-CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylmalonyl-CoA mutase | - |
Propionibacterium freudenreichii subsp. shermanii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
(R)-2-methylmalonyl-CoA | 37°C, mutant H610N | Propionibacterium freudenreichii subsp. shermanii | |
| 0.024 | - |
(R)-2-methylmalonyl-CoA | 37°C, mutant H610A | Propionibacterium freudenreichii subsp. shermanii | |
| 120 | - |
(R)-2-methylmalonyl-CoA | 37°C, wild-type | Propionibacterium freudenreichii subsp. shermanii | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| adenosylcobalamin | - |
Propionibacterium freudenreichii subsp. shermanii | |
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Release 2023.1 (January 2023)
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