General Stability | Organism |
---|---|
repeated freezing and thawing results in a gradual loss of activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-2-methyl-3-oxopropanoyl-CoA | substrate inhibition | Homo sapiens | |
Ca2+ | at high concentrations | Homo sapiens | |
Mg2+ | at high concentrations | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
(R)-2-methyl-3-oxopropanoyl-CoA | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cl- | stimulates | Homo sapiens | |
PO43- | required, maximal activity at 50 mM | Homo sapiens | |
SO42- | stimulates | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77500 | - |
2 * 77500, SDS-PAGE | Homo sapiens |
150000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 1.5 mg protein /ml in 50 mM potassium phosphate, pH 7.4, stable for at least 6 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-Methyl-3-oxopropanoyl-CoA | - |
Homo sapiens | ? | - |
? | |
(R)-2-methylmalonyl-CoA | - |
Homo sapiens | succinyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 77500, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
15 min, stable below | Homo sapiens |
55 | - |
t1/2: 3 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cobamide | one molecule of enzyme contains about 2 molecules of adenosylcobalamin | Homo sapiens |