Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dithionitrobenzoate | irreversibly inactivates | Escherichia coli | |
iodoacetate | irreversibly inactivates | Escherichia coli | |
p-chloromercuribenzoate | irreversibly inactivates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isolation of virtually homogeneous tRNA pseudouridine synthase I from strains of Escherichia coli transformed with plasmid in which the production of tRNA pseudouridine synthase I is amplified 20fold | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C or 2°C, 50% w/v glycerol, 50% loss of activity after 2 years | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine38-40 | purified tRNA pseudouridine synthase I modifies all of the hisT isoacceptors of tRNAHis, tRNATyr, and tRNALeu to products which are chromatographically indistinguishable from the respective wild-type species. These three groups of isoacceptors contain all the known topological sites for pseudouridine modification of residues 38,39, and 40 | Escherichia coli | tRNA pseudouridine38-40 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
hisT | - |
Escherichia coli |
pseudouridine synthase I | - |
Escherichia coli |