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Literature summary for 5.4.99.12 extracted from

  • Green, C.; Kammen, H.; Penhoet, E.
    Purification and properties of a mammalian tRNA pseudouridine synthase (1982), J. Biol. Chem., 257, 3045-3052.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information a thiol is required for maximum activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Calf thymus DNA native and denatured, about 66% residual activity Bos taurus
poly(rA)n 71% residual acivity Bos taurus
poly(rC)n 93% residual acivity Bos taurus
poly(rG)n 3.2% residual acivity Bos taurus
poly(rI)n 11.9% residual acivity Bos taurus
poly(rU)n 44% residual acivity Bos taurus
tRNA activity is inhibited by single tRNAs or bulk tRNA from all sources tested, and by ribosomal RNA Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information a monovalent cation is required for maximum activity Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
steer
-

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Salmonella typhimurium HisT- tRNALeu tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ?
-
?
Salmonella typhimurium HisT- tRNAPhe2 uridine39 tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3’ side of the anticodon region of approximately half of the cellular tRNAs Bos taurus Salmonella typhimurium HisT- tRNAPhe2 pseudouridine39 specific modification of uridine39 ?
Salmonelly typhimurium HisT- tRNATyr tRNAPhe2 from mutant HisT- strain defective for tRNA pseudouridine synthase I that forms pseudouridine in the 3'-side of the anticodon region of approximately half of the cellular tRNAs Bos taurus ?
-
?

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors required Bos taurus