Cloned (Comment) | Organism |
---|---|
wild-type enzyme and gabaculine-resistant mutant enzyme, overexpression in Escherichia coli | Synechococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gabaculine-resistant mutant enzyme with a 3 times lower catalytic efficiency and impaired prototropic rearrangement and transaldimination | Synechococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gabaculine | wild-type enzyme is inhibited, gabaculine-resistant mutant enzyme is not inhibited | Synechococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
(S)-4-Amino-5-oxopentanoate | gabaculine-resistant mutant enzyme | Synechococcus sp. | |
0.046 | - |
(S)-4-Amino-5-oxopentanoate | wild-type enzyme | Synechococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus sp. | - |
wild-type strain and gabaculine-resistant strain | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and gabaculine-resistant mutant enzyme | Synechococcus sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-glutamate 1-semialdehyde = 5-aminolevulinate | wild-type enzyme and gabaculine-resistant mutant enzyme use a ping-pong bi-bi mechanism in which 4,5-diaminovalerate is a likely intermediate | Synechococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-Amino-5-oxopentanoate | - |
Synechococcus sp. | 5-Amino-4-oxopentanoate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
(S)-4-Amino-5-oxopentanoate | gabaculine-resistant mutant enzyme | Synechococcus sp. | |
0.47 | - |
(S)-4-Amino-5-oxopentanoate | wild-type enzyme | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B6 | wild-type enzyme and gabaculine-resistant mutant enzyme contain vitamin B6 | Synechococcus sp. |