Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | 20 mM, 57% inhibition | Hordeum vulgare | |
cycloserine | 20 mM, 43% inhibition | Hordeum vulgare | |
D-penicillamine | 20 mM, 9% inhibition | Hordeum vulgare | |
glyoxylate | 20 mM, 27% inhibition | Hordeum vulgare | |
additional information | no inhibition by 3-hydroxyglutamate | Hordeum vulgare | |
p-chloromercuribenzoate | 0.1 mM, 45% inhibition | Hordeum vulgare | |
pyridoxal 5'-phosphate | 5 mM, 47% inhibition | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
(S)-4-Amino-5-oxopentanoate | - |
Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | mature | Hordeum vulgare | 9507 | - |
plastid | low specific activity in etiolated plastids compared to greening plastids | Hordeum vulgare | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE | Hordeum vulgare |
500000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-Amino-5-oxopentanoate | Hordeum vulgare | the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | greening | Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-Amino-5-oxopentanoate | - |
Hordeum vulgare | 5-Amino-4-oxopentanoate | - |
? | |
(S)-4-Amino-5-oxopentanoate | the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Hordeum vulgare |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
14 | 30 | 14°C: 41% of maximal activity, 22°C: 76.4% of maximal activity | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
broad | Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.8 | pH 6.7: about 45% of maximal activity, pH 8.8: about 90% of maximal activity | Hordeum vulgare |