Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.2.8 extracted from

  • Schramm, A.M.; Mehra-Chaudhary, R.; Furdui, C.M.; Beamer, L.J.
    Backbone flexibility, conformational change, and catalysis in a phosphohexomutase from Pseudomonas aeruginosa (2008), Biochemistry, 47, 9154-9162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme P368G, hanging drop vapour diffusion method Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
E325A mutant shows 0.08% of wild type activity Pseudomonas aeruginosa
P368A mutant shows 10% of wild type activity Pseudomonas aeruginosa
P368G mutant shows 8.7% of wild type activity Pseudomonas aeruginosa
R262A mutant shows 6.1% of wild type activity Pseudomonas aeruginosa
R262A/P368G mutant shows 2.5% of wild type activity Pseudomonas aeruginosa
S369A mutant shows 20.5% of wild type activity Pseudomonas aeruginosa
Y17A mutant shows 0.35% of wild type activity Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0272
-
alpha-D-glucose 1-phosphate wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0354
-
alpha-D-glucose 1-phosphate mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0366
-
alpha-D-glucose 1-phosphate mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0489
-
alpha-D-glucose 1-phosphate mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0491
-
alpha-D-glucose 1-phosphate mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0493
-
alpha-D-glucose 1-phosphate mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0518
-
alpha-D-glucose 1-phosphate mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.0667
-
alpha-D-glucose 1-phosphate mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P26276
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
Pseudomonas aeruginosa alpha-D-glucose 6-phosphate
-
?
alpha-D-mannose 1-phosphate
-
Pseudomonas aeruginosa alpha-D-mannose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
phosphomannomutase/phosphoglucomutase
-
Pseudomonas aeruginosa
PMM/PGM bifunctional enzyme Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
alpha-D-glucose 1-phosphate mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.05
-
alpha-D-glucose 1-phosphate mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.48
-
alpha-D-glucose 1-phosphate mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
0.87
-
alpha-D-glucose 1-phosphate mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
1.02
-
alpha-D-glucose 1-phosphate mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
1.23
-
alpha-D-glucose 1-phosphate mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
2.17
-
alpha-D-glucose 1-phosphate mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa
7.83
-
alpha-D-glucose 1-phosphate wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 Pseudomonas aeruginosa