Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme P368G, hanging drop vapour diffusion method | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E325A | mutant shows 0.08% of wild type activity | Pseudomonas aeruginosa |
P368A | mutant shows 10% of wild type activity | Pseudomonas aeruginosa |
P368G | mutant shows 8.7% of wild type activity | Pseudomonas aeruginosa |
R262A | mutant shows 6.1% of wild type activity | Pseudomonas aeruginosa |
R262A/P368G | mutant shows 2.5% of wild type activity | Pseudomonas aeruginosa |
S369A | mutant shows 20.5% of wild type activity | Pseudomonas aeruginosa |
Y17A | mutant shows 0.35% of wild type activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0272 | - |
alpha-D-glucose 1-phosphate | wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0354 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0366 | - |
alpha-D-glucose 1-phosphate | mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0489 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0491 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0493 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0518 | - |
alpha-D-glucose 1-phosphate | mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.0667 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P26276 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-glucose 6-phosphate | - |
? | |
alpha-D-mannose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-mannose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphomannomutase/phosphoglucomutase | - |
Pseudomonas aeruginosa |
PMM/PGM | bifunctional enzyme | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
alpha-D-glucose 1-phosphate | mutant enzyme E325A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.05 | - |
alpha-D-glucose 1-phosphate | mutant enzyme Y17A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.48 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A/P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
0.87 | - |
alpha-D-glucose 1-phosphate | mutant enzyme R262A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
1.02 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
1.23 | - |
alpha-D-glucose 1-phosphate | mutant enzyme P368G, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
2.17 | - |
alpha-D-glucose 1-phosphate | mutant enzyme S369A, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa | |
7.83 | - |
alpha-D-glucose 1-phosphate | wild type enzyme, at 25°C in 50 mM MOPS (pH 7.4) with 1 mM dithiothreitol, 1.5 mM MgSO4 | Pseudomonas aeruginosa |