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Literature summary for 5.4.2.8 extracted from
- The reaction of phosphohexomutase from Pseudomonas aeruginosa: structural insights into a simple processive enzyme (2006), J. Biol. Chem., 281, 15564-15571.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| phospho- and dephospho-enzyme in complex with reaction intermediate glucose 1,6-bisphosphate at 1.9 and 2.0 A | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N110A | no remarkable differences in Km and Vmax value compared to wild-type, but intermediate glucose-1,6-bisphosphate dissociates from mutant 25times more often than from wild-type | Pseudomonas aeruginosa |
| R15A | no remarkable differences in Km and Vmax value compared to wild-type, but intermediate glucose-1,6-bisphosphate dissociates from mutant 25times more often than from wild-type | Pseudomonas aeruginosa |
| R20A | no catalytic activity | Pseudomonas aeruginosa |
| R241C | 0.3% of wild-type acivity, with Km value similar to wild-type | Pseudomonas aeruginosa |
| R247A | no remarkable differences in Km and Vmax value compared to wild-type, modest increase in dissociation of intermediate glucose-1,6-bisphosphate from enzyme | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0127 | - |
alpha-D-glucose 1-phosphate | mutant RN110A | Pseudomonas aeruginosa |  |
| 0.0132 | - |
alpha-D-glucose 1-phosphate | mutant R15A | Pseudomonas aeruginosa | |
| 0.0195 | - |
alpha-D-glucose 1-phosphate | mutant R247A | Pseudomonas aeruginosa | |
| 0.0273 | - |
alpha-D-glucose 1-phosphate | wild-type | Pseudomonas aeruginosa | |
| 0.0279 | - |
alpha-D-glucose 1-phosphate | mutant R421C | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
bifunctional phosphomannomutase/phosphoglucomutase | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | - |
Pseudomonas aeruginosa | alpha-D-glucose 6-phosphate | - |
? | |
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