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Literature summary for 5.4.2.7 extracted from

  • Rashid, N.; Imanaka, H.; Fukui, T.; Atomi, H.; Imanaka, T.
    Presence of a novel phosphopentomutase and a 2-deoxyribose 5-phosphate aldolase reveals a metabolic link between pentoses and central carbon metabolism in the hyperthermophilic archaeon Thermococcus kodakaraensis (2004), J. Bacteriol., 186, 4185-4191.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.2.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 2-deoxy-D-ribose 1-phosphate 90°C Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1 mM, high activation Thermococcus kodakarensis
Mn2+ 1 mM, 50% of the activation with Mg2+ Thermococcus kodakarensis
Ni2+ 1 mM, 70% of the activation with Mg2+ Thermococcus kodakarensis
Zn2+ 1 mM, 45% of the activation with Mg2+ Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
210000
-
 gel filtration Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q6I7B6 KOD1
-

Purification (Commentary)

Purification (Comment) Organism
partial Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
210
-
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-ribose 1-phosphate
-
 Thermococcus kodakarensis 2-deoxy-D-ribose 5-phosphate
-
 ?
additional information trace levels of activity with glucose 1-phosphate and mannose 1-phosphate. No activity with fructose 1-phosphate, N-acetylglucosamine 1-phosphate and 3-phosphoglycerate Thermococcus kodakarensis ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
PPM
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermococcus kodakarensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 55% of maximal activity, 100°C: about 90% of maximal activity Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 half-life: 90 min Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
173
-
 2-deoxy-D-ribose 1-phosphate 90°C Thermococcus kodakarensis