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Literature summary for 5.4.2.2 extracted from
- Heterologous expression and biochemical characterization of recombinant alpha phosphoglucomutase from Mycobacterium tuberculosis H37Rv (2012), Protein Expr. Purif., 85, 117-124.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pgmA, sequence comparison, phylogenetic analysis and expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 2,6-diphosphate | noncompetitive inhibitor | Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Mycobacterium tuberculosis | |
| 0.1 | 2 | alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis | - |
D-glucose 6-phosphate | - |
r | |
| alpha-D-glucose 1-phosphate | Mycobacterium tuberculosis H37Rv | - |
D-glucose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | I6Y2G3 | gene pgmA | - |
| Mycobacterium tuberculosis H37Rv | I6Y2G3 | gene pgmA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, protein refolding, and ultrafiltration | Mycobacterium tuberculosis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) inclusion bodies | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67.5 | - |
purified recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis | D-glucose 6-phosphate | - |
r | |
| alpha-D-glucose 1-phosphate | - |
Mycobacterium tuberculosis H37Rv | D-glucose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | four-domain architecture, homology modeling, overview | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha phosphoglucomutase | - |
Mycobacterium tuberculosis |
| PGM | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | recombinant enzyme | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 70.31 | - |
alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mycobacterium tuberculosis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.4 | purified recombinant enzyme, stable at | Mycobacterium tuberculosis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | structure homology modeling, overview | Mycobacterium tuberculosis |
| physiological function | the enzyme plays an important role in polysaccharide capsule formation and virulence in a number of bacterial pathogens | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 58.4 | - |
alpha-D-glucose 1-phosphate | recombinant enzyme, pH 7.4, 30°C | Mycobacterium tuberculosis | |
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