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Literature summary for 5.4.2.2 extracted from

  • Mitra, S.; Cui, J.; Robbins, P.W.; Samuelson, J.
    A deeply divergent phosphoglucomutase (PGM) of Giardia lamblia has both PGM and phosphomannomutase activities (2010), Glycobiology, 20, 1233-1240.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.4.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Eascherichia coli and Saccharomyces cerevisiae BY4741 wild type and pgm1DELTA/pgm2DELTA mutant strain Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 alpha-D-glucose 1-phosphate PGM2, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
0.0082
-
 alpha-D-glucose 1-phosphate PGM1, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose affinity column chromatography Giardia intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76
-
 PGM2, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis
87
-
 PGM1, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES, pH 7.1, 5 mM MgCl2, at 30°C Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate
-
 Giardia intestinalis alpha-D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PGM1 PGM1 has phosphoglucomutase activity only Giardia intestinalis
PGM2 the enzyme from Giardia lamblia has both phosphoglucomutase and phosphomannomutase activities Giardia intestinalis