Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
crystals of iPGM complexed with 3-phosphoglycerate and Mn2+ at 1.9 A resolution | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D12N | no activity | Geobacillus stearothermophilus |
H123N | 3% of wild-type activity | Geobacillus stearothermophilus |
H125N | 67% of wild-type activity | Geobacillus stearothermophilus |
H128N | 20% of wild-type activity | Geobacillus stearothermophilus |
H407N | no activity | Geobacillus stearothermophilus |
H42N | 91% of wild-type activity | Geobacillus stearothermophilus |
H42N/H128N | 8% of wild-type activity | Geobacillus stearothermophilus |
H445N | 18% of wild-type activity | Geobacillus stearothermophilus |
H462N | 0.3% of wild-type activity | Geobacillus stearothermophilus |
H66N | 2% of wild-type activity | Geobacillus stearothermophilus |
R261L | no activity | Geobacillus stearothermophilus |
S62A | no activity | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | absolutely required for activity, maximal activation at 1 mM | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Geobacillus stearothermophilus | - |
3-phospho-D-glycerate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q9X519 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant iPGM | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Geobacillus stearothermophilus | 3-phospho-D-glycerate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
iPGM | - |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 2% and 22% of maximal activity at pH 6.0 and pH 7.0, respectively, at 1 mM Mn2+ | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2,3-bisphosphoglycerare independend PGM | Geobacillus stearothermophilus |