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Literature summary for 5.3.3.1 extracted from
- Refolding strategies for ketosteroid isomerase following insoluble expression in Escherichia coli (2006), Biotechnol. Bioeng., 94, 1089-1098.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of C-terminally His6-tagged enzyme as insoluble protein in inclusion bodies in Escherichia coli strain BLR(DE3) | Comamonas testosteroni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Androstene-3,17-dione | Comamonas testosteroni | - |
4-Androstene-3,17-dione | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | P00947 | i.e. Pseudomonas testosteroni | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BLR(DE3) to homogeneity by nickel affinity chromatography after dilution refolding, method optimization, overview | Comamonas testosteroni |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| 70-87% refolding of recombinant C-terminally His6-tagged enzyme after expression in Escherichia coli as insoluble protein and solubilization by denaturation, detergent treatment, and 40fold dilution by a single-step column-based refolding step with an elution gradient, dilution with buffer 1% bovine serum albumin, 20 mM phosphate, 150 mM NaCl, optimal at pH 6.8, method optimization, overview | Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30600 | - |
purified diluted recombinant enzyme | Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-Androstene-3,17-dione | - |
Comamonas testosteroni | 4-Androstene-3,17-dione | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA5-3-ketosteroid isomerase | - |
Comamonas testosteroni |
| ketosteroid isomerase | - |
Comamonas testosteroni |
| KSI | - |
Comamonas testosteroni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Comamonas testosteroni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Comamonas testosteroni |
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Release 2023.1 (January 2023)
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