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Literature summary for 5.3.3.1 extracted from

  • Thomas, J.L.; Berko, E.A.; Faustino, A.; Myers, R.P.; Strickler, R.C.
    Human placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5-->4-ene-isomerase: purification from microsomes, substrate kinetics, and inhibition by product steroids (1988), J. Steroid Biochem., 31, 785-793.
    View publication on PubMed
Search for more literature for 5.3.3.1

Inhibitors

Inhibitors Comment Organism Structure
5-androstene-3,17-dione competitive towards 5-pregnene-3,20-dione Homo sapiens
5-Pregnene-3,20-dione competitive inhibitor towards 5-androstene-3,17-dione Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0097
-
 5-Pregnene-3,20-dione
-
 Homo sapiens
0.0237
-
 5-androstene-3,17-dione
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 4 * 19000, enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase, SDS-PAGE Homo sapiens
76000
-
 enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens in pregnancy placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5--4 isomerase produce progesterone from pregnenolone and metabolize fetal dehydroepiandrosterone sulfate to androstenedione, an estrogen precursor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and Steroid DELTA-isomerase
-

Purification (Commentary)

Purification (Comment) Organism
enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5 to 4 isomerase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Androstene-3,17-dione
-
 Homo sapiens 4-Androstene-3,17-dione
-
 ?
5-Pregnene-3,20-dione
-
 Homo sapiens 4-Pregnene-3,20-dione
-
 ?
additional information in pregnancy placental 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5--4 isomerase produce progesterone from pregnenolone and metabolize fetal dehydroepiandrosterone sulfate to androstenedione, an estrogen precursor Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 19000, enzyme complex of 3beta-hydroxy-5-ene-steroid dehydrogenase and steroid DELTA-isomerase, SDS-PAGE Homo sapiens