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Literature summary for 5.3.1.9 extracted from
- Biochemical characterization of recombinant phosphoglucose isomerase of Mycobacterium tuberculosis (2005), Biochem. Biophys. Res. Commun., 337, 626-632.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| gluconate 6-phosphate | - |
Mycobacterium tuberculosis |  |
| additional information | not inhibitory: EDTA | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.318 | - |
D-fructose 6-phosphate | pH 7.6, 22°C | Mycobacterium tuberculosis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Mycobacterium tuberculosis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for Na+, K+, Mg2+, Ca2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
2 * 61450, mass spectrometry, 2 * 61000, SDS-PAGE | Mycobacterium tuberculosis |
| 61450 | - |
2 * 61450, mass spectrometry, 2 * 61000, SDS-PAGE | Mycobacterium tuberculosis |
| 120000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
expression in Escherichia coli | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 617.7 | - |
pH 7.6, 22°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | - |
Mycobacterium tuberculosis | D-fructose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 61450, mass spectrometry, 2 * 61000, SDS-PAGE | Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, no loss of activity | Mycobacterium tuberculosis |
| 55 | - |
30 min, complete inactivation | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Mycobacterium tuberculosis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
gluconate 6-phosphate | pH 7.6, 22°C | Mycobacterium tuberculosis | |
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