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Literature summary for 5.3.1.9 extracted from
- Novel type of glucose-6-phosphate isomerase in the hyperthermophilic archaeon Pyrococcus furiosus (2001), J. Bacteriol., 183, 3428-3435.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-fructose 6-phosphate | 80°C, pH 7.0, native enzyme | Pyrococcus furiosus |  |
| 1.2 | - |
D-fructose 6-phosphate | 80°C, pH 7.0, recombinant enzyme | Pyrococcus furiosus | |
| 7.9 | - |
D-glucose 6-phosphate | 80°C, pH 7.0, recombinant enzyme | Pyrococcus furiosus | |
| 8.7 | - |
D-glucose 6-phosphate | 80°C, pH 7.0, native enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
2 * 23000, SDS-PAGE | Pyrococcus furiosus |
| 41000 | - |
native enzyme, gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
DSM 3638 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34.8 | - |
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate | - |
Pyrococcus furiosus | D-fructose 6-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 96 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
pH 7.0, 180 min, about 10% loss of activity | Pyrococcus furiosus |
| 80 | - |
pH 7.0, 180 min, about 20% loss of activity | Pyrococcus furiosus |
| 90 | - |
pH 7.0, 180 min, about 50% loss of activity | Pyrococcus furiosus |
| 100 | - |
pH 7.0, 180 min, about 60% loss of activity | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | about 50% of maximal activity above pH 6.0 and below pH 8.0 | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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