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Literature summary for 5.3.1.9 extracted from

  • Kanno, H.; Fujii, H.; Miwa, S.
    Expression and enzymatic characterization of human glucose phosphate isomerase (GPI) variants accounting for GPI deficiency (1998), Blood Cells Mol. Dis., 24, 54-61.
    View publication on PubMed
Search for more literature for 5.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
native enzyme and mutant enzymes T5I, T224M, Q343R, D539N Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q343R mutant enzymes Thr5 to Ile exhibits marked thermal instability. Mutant Thr224 to Met shows normal substrate affinity in spite of slight decrease in both specific activity and thermostability. Mutant Gln343 to Arg and Asp539 to Asn show impaired substrate affinity Homo sapiens
R539N mutant enzymes Thr5 to Ile exhibits marked thermal instability. Mutant Thr224 to Met shows normal substrate affinity in spite of slight decrease in both specific activity and thermostability. Mutant Gln343 to Arg and Asp539 to Asn show impaired substrate affinity Homo sapiens
T224M mutant enzymes Thr5 to Ile exhibits marked thermal instability. Mutant Thr224 to Met shows normal substrate affinity in spite of slight decrease in both specific activity and thermostability. Mutant Gln343 to Arg and Asp539 to Asn show impaired substrate affinity Homo sapiens
T5I mutant enzymes Thr5 to Ile exhibits marked thermal instability. Mutant Thr224 to Met shows normal substrate affinity in spite of slight decrease in both specific activity and thermostability. Mutant Gln343 to Arg and Asp539 to Asn show impaired substrate affinity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0596
-
 fructose 6-phosphate mutant enzyme Thr224 to Met Homo sapiens
0.0635
-
 fructose 6-phosphate native enzyme Homo sapiens
0.0657
-
 fructose 6-phosphate mutant enzyme Thr5 to Ile Homo sapiens
0.0734
-
 fructose 6-phosphate mutant enzyme Asp539 to Asn Homo sapiens
0.0769
-
 fructose 6-phosphate mutant enzyme Gln343 to Arg Homo sapiens
0.351
-
 glucose 6-phosphate mutant enzyme Thr224 to Met Homo sapiens
0.445
-
 glucose 6-phosphate native enzyme Homo sapiens
0.449
-
 glucose 6-phosphate mutant enzyme Thr5 to Ile Homo sapiens
0.505
-
 glucose 6-phosphate mutant enzyme Asp539 to Asn Homo sapiens
0.573
-
 glucose 6-phosphate mutant enzyme Gln343 to Arg Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 native enzyme and mutant enzymes Thr5 to Ile, Thr224 to Met, Gln343 to Arg, and Asp539 to Asn
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fructose 6-phosphate r Homo sapiens Glucose 6-phosphate
-
 ?
Glucose 6-phosphate r Homo sapiens Fructose 6-phosphate
-
 ?