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Literature summary for 5.3.1.6 extracted from
- Substrate specificity of ribose-5-phosphate isomerases from Clostridium difficile and Thermotoga maritima (2010), Biotechnol. Lett., 32, 829-835.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridioides difficile |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular dockng using L-xylose and D-ribose as the substrate. Residue M95 seems to be a determinant residue for the specificity on free sugars | Clostridioides difficile |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is not affected by metal ions and not inhibited by EDTA | Clostridioides difficile |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5 | 20 | L-talose | pH 7.5, 40°C | Clostridioides difficile |  |
| 30 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile | |
| 270 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
| 460 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17200 | - |
2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
| 17217 | - |
2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
| 34000 | - |
gel filtration | Clostridioides difficile |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Clostridioides difficile | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | ? | - |
? | |
| additional information | Clostridioides difficile ATCC BAA-1382D-5 | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridioides difficile | - |
- |
- |
| Clostridioides difficile ATCC BAA-1382D-5 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
substrate D-sorbose, pH 7.5, 40°C | Clostridioides difficile |
| 3 | - |
substrate D-psicose, pH 7.5, 40°C | Clostridioides difficile |
| 7 | - |
substrate L-lyxose, pH 7.5, 40°C | Clostridioides difficile |
| 9 | - |
substrate L-fructose, pH 7.5, 40°C | Clostridioides difficile |
| 14 | - |
substrate D-ribulose, pH 7.5, 40°C | Clostridioides difficile |
| 15 | - |
substrate D-allose, pH 7.5, 40°C | Clostridioides difficile |
| 28 | - |
substrate L-talose, pH 7.5, 40°C | Clostridioides difficile |
| 45 | - |
substrate L-talose, pH 7.5, 40°C | Clostridioides difficile |
| 53 | - |
substrate D-ribose, pH 7.5, 40°C | Clostridioides difficile |
| 57 | - |
substrate L-xylulose, pH 7.5, 40°C | Clostridioides difficile |
| 120 | - |
substrate D-gulose, pH 7.5, 40°C | Clostridioides difficile |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Clostridioides difficile | D-psicose | - |
r | |
| D-allose | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-psicose | - |
r | |
| D-gulose | - |
Clostridioides difficile | D-sorbose | - |
r | |
| D-psicose | - |
Clostridioides difficile | D-allose | - |
r | |
| D-ribose | - |
Clostridioides difficile | D-ribulose | - |
r | |
| D-ribose | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-ribulose | - |
r | |
| D-ribose 5-phosphate | - |
Clostridioides difficile | D-ribulose 5-phosphate | - |
? | |
| D-ribose 5-phosphate | - |
Clostridioides difficile ATCC BAA-1382D-5 | D-ribulose 5-phosphate | - |
? | |
| D-ribulose | - |
Clostridioides difficile | D-ribose | - |
r | |
| D-sorbose | - |
Clostridioides difficile | D-gulose | - |
r | |
| L-fructose | - |
Clostridioides difficile | L-mannose | - |
r | |
| L-lyxose | - |
Clostridioides difficile | L-xylulose | - |
r | |
| L-lyxose | - |
Clostridioides difficile ATCC BAA-1382D-5 | L-xylulose | - |
r | |
| L-tagatose | - |
Clostridioides difficile | L-talose | - |
r | |
| L-talose | - |
Clostridioides difficile | L-tagatose | - |
r | |
| L-xylulose | - |
Clostridioides difficile | L-lyxose | - |
r | |
| additional information | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile | ? | - |
? | |
| additional information | enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose. The specific activity decreases in the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose | Clostridioides difficile ATCC BAA-1382D-5 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17217, calculated, 2 * 17200, SD-PAGE | Clostridioides difficile |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RpiB | - |
Clostridioides difficile |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Clostridioides difficile |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life 53 min | Clostridioides difficile |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 140 | - |
L-talose | pH 7.5, 40°C | Clostridioides difficile | |
| 160 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
| 200 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile | |
| 15100 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Clostridioides difficile |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridioides difficile | isoelectric focusing | - |
6.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
L-talose | pH 7.5, 40°C | Clostridioides difficile | |
| 0.4 | - |
D-allose | pH 7.5, 40°C | Clostridioides difficile | |
| 0.6 | - |
D-ribose | pH 7.5, 40°C | Clostridioides difficile | |
| 500 | - |
D-ribose 5-phosphate | pH 7.5, 40°C | Clostridioides difficile | |
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