Application | Comment | Organism |
---|---|---|
synthesis | RpiB is a potential producer of L-form monosaccharides | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not activated by monovalent or divalent cations | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23724 | - |
4 * 23724, calculated from amino acid sequence | Streptococcus pneumoniae |
24000 | - |
4 * 24000, SDS-PAGE | Streptococcus pneumoniae |
96000 | - |
gel filtration | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribose 5-phosphate | Streptococcus pneumoniae | highest specific activity | D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration | Streptococcus pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
using D-sorbose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0011 | - |
using L-fructose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0013 | - |
using L-tagatose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0019 | - |
using D-allose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0021 | - |
using D-psicose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0025 | - |
using D-ribose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0053 | - |
using D-gulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.0096 | - |
using L-lyxose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.019 | - |
using L-talose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.027 | - |
using L-lyxose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.048 | - |
using D-ribulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.058 | - |
using D-glucose 6-phosphate as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.121 | - |
using L-xylulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
0.388 | - |
using D-ribose 5-phosphate as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Streptococcus pneumoniae | D-psicose | - |
r | |
D-Glucose 6-phosphate | - |
Streptococcus pneumoniae | ? | - |
? | |
D-gulose | - |
Streptococcus pneumoniae | D-sorbose | - |
r | |
D-psicose | - |
Streptococcus pneumoniae | D-allose | - |
r | |
D-ribose | - |
Streptococcus pneumoniae | D-ribulose | - |
r | |
D-ribose 5-phosphate | highest specific activity | Streptococcus pneumoniae | D-ribulose 5-phosphate | - |
? | |
D-ribulose | - |
Streptococcus pneumoniae | D-ribose | - |
r | |
D-sorbose | lowest activity with L-sorbose | Streptococcus pneumoniae | D-gulose | - |
r | |
L-fructose | - |
Streptococcus pneumoniae | L-mannose | - |
r | |
L-lyxose | particularly high activity with L-lyxose | Streptococcus pneumoniae | L-xylulose | - |
r | |
L-Mannose | - |
Streptococcus pneumoniae | L-Fructose | - |
r | |
L-tagatose | particularly high activity with L-tagatose | Streptococcus pneumoniae | L-talose | - |
r | |
L-talose | particularly high activity with L-talose | Streptococcus pneumoniae | L-tagatose | - |
r | |
L-xylulose | particularly high activity with L-lyxose | Streptococcus pneumoniae | L-lyxose | - |
r | |
additional information | no activity is observed with D-mannose-6-phosphate | Streptococcus pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 24000, SDS-PAGE | Streptococcus pneumoniae |
homotetramer | 4 * 23724, calculated from amino acid sequence | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
ribose-5-phosphate isomerase | - |
Streptococcus pneumoniae |
RpiB | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Streptococcus pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | - |
Streptococcus pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | enzyme activity is stable for 48 h at 35°C, and shows half-lives of 15 h at 40°C, and 6 h at 50°C | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptococcus pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Streptococcus pneumoniae |