Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
C65A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
D8A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
H133A | the mutant shows 15% activity compared to the wild type enzyme | Acetivibrio thermocellus |
H98A | the mutant shows 13% activity compared to the wild type enzyme | Acetivibrio thermocellus |
H9A | the mutant shows 63% activity compared to the wild type enzyme | Acetivibrio thermocellus |
N99A | the mutant shows 35% activity compared to the wild type enzyme | Acetivibrio thermocellus |
R132A | the mutant exhibits an increase in D-psicose isomerization activity (116% activity compared to the wild type enzyme) | Acetivibrio thermocellus |
R132D | the mutant shows increased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
R132E | the mutant shows increased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
R132E | the specific activity and catalytic efficiency (kcat/Km) of the R132E mutant for D-psicose are 1.4 and 1.5fold higher than those of the wild type enzyme, respectively | Acetivibrio thermocellus |
R132I | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
R132K | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
R132Q | the mutant shows decreased catalytic efficiency toward D-psicose compared to the wild type enzyme | Acetivibrio thermocellus |
R136A | the mutant shows 15% activity compared to the wild type enzyme | Acetivibrio thermocellus |
T135A | the mutant shows 91% activity compared to the wild type enzyme | Acetivibrio thermocellus |
T67A | the mutant shows 46% activity compared to the wild type enzyme | Acetivibrio thermocellus |
Y42A | the mutant shows no activity for D-psicose | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus | |
46 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
53 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
53 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
54 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
56 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
78 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | A3DIL8 | - |
- |
Acetivibrio thermocellus ATCC 27405 | A3DIL8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography | Acetivibrio thermocellus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.259 | - |
mutant enzyme H98A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
0.299 | - |
mutant enzyme H133A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
0.299 | - |
mutant enzyme R136A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
0.698 | - |
mutant enzyme N99A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
0.917 | - |
mutant enzyme T67A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
1.256 | - |
mutant enzyme H9A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
1.815 | - |
mutant enzyme T135A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
1.994 | - |
wild type enzyme, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
2.313 | - |
mutant enzyme R132A, using D-psicose as substrate, pH 7.5, 80°C | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | the conversion yield of D-psicose to D-allose is 32% for the R132E mutant enzyme and 25% for the wild type enzyme after 80 min | Acetivibrio thermocellus | D-allose | - |
r | |
D-psicose | the conversion yield of D-psicose to D-allose is 32% for the R132E mutant enzyme and 25% for the wild type enzyme after 80 min | Acetivibrio thermocellus ATCC 27405 | D-allose | - |
r | |
D-ribulose 5-phosphate | - |
Acetivibrio thermocellus | D-ribose 5-phosphate | - |
r | |
D-ribulose 5-phosphate | - |
Acetivibrio thermocellus ATCC 27405 | D-ribose 5-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CTRpiB | - |
Acetivibrio thermocellus |
ribose-5-phosphate isomerase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Acetivibrio thermocellus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | - |
Acetivibrio thermocellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | the half-lives of the wild type enzyme at 60°C, 65°C, 70°C, 75°C, and 80°C are 11, 7.0, 4.2, 1.5, and 0.6 h, respectively | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1962 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
2211 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus | |
2335 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
2347 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
2580 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
2674 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
2743 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acetivibrio thermocellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
D-psicose | mutant enzyme R132I, pH 7.5, 80°C | Acetivibrio thermocellus | |
37 | - |
D-psicose | mutant enzyme R132K, pH 7.5, 80°C | Acetivibrio thermocellus | |
43 | - |
D-psicose | mutant enzyme R132Q, pH 7.5, 80°C | Acetivibrio thermocellus | |
44 | - |
D-psicose | wild type enzyme, pH 7.5, 80°C | Acetivibrio thermocellus | |
46 | - |
D-psicose | mutant enzyme R132A, pH 7.5, 80°C | Acetivibrio thermocellus | |
58 | - |
D-psicose | mutant enzyme R132D, pH 7.5, 80°C | Acetivibrio thermocellus | |
64 | - |
D-psicose | mutant enzyme R132E, pH 7.5, 80°C | Acetivibrio thermocellus |